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At 336 hours the mean concentration was 0.91 ± 0.02 units/m L.The three admixtures were stored under refrigeration (2°C–8°C [36°F–46°F]), and one sample of each admixture was withdrawn and tested in duplicate at 0, 6, 24, 48, 72, 144, 168, 192, 216, 240, 312, and 336 hours.Utilizing high performance liquid chromatography, each sample underwent immediate testing.Time points were stable if the mean concentration was at least 0.80 units/m L.All time points retained at least 90% of the equilibrium concentration, with the exception of hour 168 (0.79 ± 0.03 units/m L).
Conclusion: Based on these results, regular human insulin 100 units added to 0.9% sodium chloride for injection in a polyvinylchloride bag to yield a total volume of 100 m L is stable for up to 336 hours when stored at 2°C–8°C (36°F–46°F).